Investigating Effect Essay

PlanAim The aim of the experiment is to find out what do temperature has on the perform of a peptidase enzyme on exposed develop flash.Enzymes ar biological catalysts. They be made in livings things built up by amino acids to make protein. Enzymes are able to speed up reactions and go off repeat reactions.There are various factors that affect the action at law of enzymes they areY TemperatureY pHY SpecificityY meanness of enzyme or substrateEnzymes are specific, this way of life that they only work on genius substrate molecule. A substrate molecule is what the enzyme actually plant on.The factors I have chosen to investigate are temperature. This at that placefore government agency that the temperature allow for be the independent protean.In the experiment there exit be a down(p)right plastic backing of developed spud, which provide have a black gelatin pelage on it. The gelatin coating is protein, which is the substrate molecule. I volition personate the film into protease solution, which is the enzyme. By having the gelatin coat I am able to go to what eliminates to the gelatine coat when the temperature increases. I can find out if temperature affects the action of a protease enzyme.PredictionEnzymes have an best temperature, which is generally to a lower place 400C. The optimum temperature is when enzymes works best and fas adjudicate at. When the temperature rises the rate increases. This is because the substrate and enzyme molecules are moving faster because the temperature has increased. This means that the molecules have more energy. They consequently are likely to collide more often with to each one other and a reaction will take place. barely if the temperature goes over the optimum temperature the reaction slows down and the enzyme denatures. This means that it has changed shape and therefore the substrate can no protracted give way into the enzyme.The diagram below shows how the substrate molecules which is pr otein fits into the enzyme, which is a protease molecule. This type of mechanism is called the lock and key hypothesis.If the active site, which is the enzyme, is heated in like manner much it will change shape and no longer fit the substrate. The substrate therefore no longer is able to react if there is no active enzyme.I prognosticate that when the temperature increases the time taken for the gelatine to be broken down will decrease. This is because temperature is a catalyst, which helps to speed up the enzymes, which are biological catalysts. When the temperature is 300C I predict that it will take longer for the film to expire crystalline than when the film is in a temperature of 600C. However at a certain temperature in the experiment I predict that there will be an optimum temperature. This is when the enzyme works best at. later on this point the enzymes beat to slow down and eventually denature which means it is harder for the substrate molecules to fit into the enzy me molecules.As I predict that when the temperature increases the time taken for the gelatine to be broken down decreases until it reaches the optimum temperature I therefore predict that the rate of reaction will increase when the temperature increases until it reaches the point when the enzymes start to denature.When the temperature is increased the enzyme molecules will break down the black gelatine coat quicker and therefore the developed film will become transparent faster. When temperature is increased the substrate molecules of protein will collide more frequently with the enzyme molecules. So if the temperature is increased from 300C to 600C the enzyme molecule will break the black gelatine down faster to leave the transparent plastic backing.The two diagrams show the number of temperature between substrate molecules and enzyme molecules. They are only rough diagrams of what will happen between the two molecules.Y Substrate molecule-Y Enzyme molecule-Methodframe-up The set up that I am going to use for the experiment will be a riddle tube, developed film with a gelatine coat, splint, spray, stopwatch, thermometer and electric pissing baths. This equipment is suitable for this experiment because it is easily available, it is balmy to fix up up and use and it is easy to collect results with.This is how the experiment will be set upI will firstly measure the majority of protease solution by using a syringe, which will be 10cm3 and and so put it into a streamlet tube. I will then get two developed films and hook conducting wire onto each so I am able to get them out of the tube easily. The wire will be labelled so it is easy to see which film is which. I will then put the test tube into an electric piddle bath, which is at a specific temperature for example 300C. I will leave it in the bath for three minutes and then put the two films into the test tube. Every 30 seconds I will poker chip to see if the film has become transparent. When the two films have become transparent I take them out of the test tube. I then imagethe pH of the protease solution by getting a supply rod and dipping it into the solution and then put the solution onto pH paper. introductory experimentFor my preliminary experiment I set up the apparatus as above. As it was only preliminary I used one film. I chose two temperatures to put two test tubes of protease into, they were 600C and 300C. I put the two test tubes into the two different electric water baths and then after three minutes put film in each. This is how the results turned outTemperature of water bath/0CTest tube in water bath with no developed film/secsTime taken for film to become transparent/secsRate of reaction/ 1/secs (S-1)301808000.0013601803000.0033This table of results indicates that when the temperature increases the time taken for the film to become transparent is less. It withal shows that when the temperature increases that rate of reaction also increases until it reaches t he optimum temperature.This is what I expect will happen to the results in my final experiment.VariablesIn this experiment the independent variable will be the temperature, the dependent variable will be the time it takes for the films to become transparent and the controls areY Concentration of proteaseY Volume of ProteaseY painting sizeThe experiment should be carried out the same for each test tube and the pH should stay the same for all test tubes. The assiduousness of the protease solution will be 0.5% and the volume of each protease solution will be 10cm3.RangeThe range of temperatures that I am going to use will be 300C, 400C, 500C, 600C, 700C.If I have a temperature any higher than 700C the enzyme would most probably denature. I havent got a temperature any lower than 300C because it would take too long for the gelatine to break down in the time given.ReliabilityIn my final experiment I am going to use a syringe to measure out the volume of protease needed. A syringe is unblemished enough for this experiment.I will put two developed films into each test tube to improve reliability of my results. I will also use a stopwatch to time when I put the films into the test tube and when to check the films. The electric water baths are really easy to use and they control the variables very precisely unlike heating the test tube with a bunsen burner, as the temperature can go jolly up and down.SafetyWhilst doing the experiment I will have my fuzz tied back, I will wear a lab coat and I will also wear safety goggles passim as I am using protease which if gets into your eyes it can be dangerous.